A fully automated microdialysis system with built-in electrochemical sensor for on-line monitoring of biologically active substances has been designed in collaboration with the Theoretical Institute of the Faculty of Medicine, Palacký University in Olomouc.
The infusion dispenser consists of a sleeve with control electronics module for wired or wireless communication with a PC or tablet, mechanical part of the infusion dispenser, stepper motor, mechanical arrangement to transfer the stepper motor’s driving force to the drive piston system and the driving piston itself. The driving piston is supplemented by attachments for a wide range of Hamilton syringes from which direct dosing of infusion solution into the system arrives.
Ingenious mechanical design and use of internal stepping of the pump ensure continuous dosing from 0.1 µl/min to 10 µl/min using 5 ml syringes. With smaller volume syringes (2.5, 1 or 0.5 ml), it is possible to achieve very good results even when continuously dosing at even lower perfusion rates.
The drive piston is provided with a detection function that allows the syringe plunger, following travel of the plunger to the working position, to utilize substantially higher speeds and thus significantly increase efficiency and speed up work with the dispenser.
The evaluation unit consists of a specially designed casing in which the control and evaluation electronics is located for the supply of power to the detection cell, transfer and assessment of measured signals and communication with the control PC.
The detection cell consists of a specially designed housing with built-in microelectrochemical sensor suitable for in vivo analysis and NO monitoring and other biologically active substances. Current output from the microelectrochemical sensor is detected by the built-in potentiostat and after amplification is sent to the evaluation unit.